【转贴】多聚甲醛配制方法

漠*

你的标本要做免疫组化，要做原位杂交，那么就不能用常规的固定方法，否则组织的抗原和核酸就会给破坏了
现在多聚甲醛固定已经很普及了，知道它的配制方法么？

1) 900 ml dd H20 + 500 ul 1N NaOH

2) Heat to 65-70℃

3) While stirring, add 40 g paraformaldehyde

4) Continue heating and stirring until paraformaldehyde is dissolved

5) Let cool to room temperature

6) Add 100 ml of 10X PBS

7) pH to 7.3 with HCl

8) Sterile with filter

9) Store at 4℃, protected from light